Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.
Identifieur interne : 004865 ( Main/Exploration ); précédent : 004864; suivant : 004866Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.
Auteurs : Pei-Lan Shao [Taïwan] ; Po-Ren Hsueh ; Luan-Yin Chang ; Chun-Yi Lu ; Chuan-Liang Kao ; Yu-Ping Chiang ; Hsiang-Yi Huang ; Fu-Yuan Huang ; Chin-Yun Lee ; Lung-Ji Chang ; T-C Wu ; Li-Min HuangSource :
- Journal of biomedical science [ 1021-7770 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Immunoglobuline G.
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Antigènes viraux, Syndrome respiratoire aigu sévère, Virus du SRAS.
- Humains, Sensibilité et spécificité, Statistiques comme sujet, Test ELISA, Tests sérologiques.
English descriptors
- KwdEn :
- Antigens, Viral (immunology), Enzyme-Linked Immunosorbent Assay (methods), Humans, Immunoglobulin G (analysis), SARS Virus (immunology), Sensitivity and Specificity, Serologic Tests (methods), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Statistics as Topic.
- MESH :
- chemical , analysis : Immunoglobulin G.
- chemical , immunology : Antigens, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- Humans, Sensitivity and Specificity, Statistics as Topic.
Abstract
Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.
DOI: 10.1007/s11373-004-8184-6
PubMed: 15864739
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002761
- to stream PubMed, to step Curation: 002761
- to stream PubMed, to step Checkpoint: 002747
- to stream Ncbi, to step Merge: 000F26
- to stream Ncbi, to step Curation: 000F26
- to stream Ncbi, to step Checkpoint: 000F26
- to stream Main, to step Merge: 004B71
- to stream Main, to step Curation: 004865
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.</title><author><name sortKey="Shao, Pei Lan" sort="Shao, Pei Lan" uniqKey="Shao P" first="Pei-Lan" last="Shao">Pei-Lan Shao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name></author><author><name sortKey="Chang, Luan Yin" sort="Chang, Luan Yin" uniqKey="Chang L" first="Luan-Yin" last="Chang">Luan-Yin Chang</name></author><author><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name></author><author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name></author><author><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name></author><author><name sortKey="Huang, Hsiang Yi" sort="Huang, Hsiang Yi" uniqKey="Huang H" first="Hsiang-Yi" last="Huang">Hsiang-Yi Huang</name></author><author><name sortKey="Huang, Fu Yuan" sort="Huang, Fu Yuan" uniqKey="Huang F" first="Fu-Yuan" last="Huang">Fu-Yuan Huang</name></author><author><name sortKey="Lee, Chin Yun" sort="Lee, Chin Yun" uniqKey="Lee C" first="Chin-Yun" last="Lee">Chin-Yun Lee</name></author><author><name sortKey="Chang, Lung Ji" sort="Chang, Lung Ji" uniqKey="Chang L" first="Lung-Ji" last="Chang">Lung-Ji Chang</name></author><author><name sortKey="Wu, T C" sort="Wu, T C" uniqKey="Wu T" first="T-C" last="Wu">T-C Wu</name></author><author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15864739</idno><idno type="pmid">15864739</idno><idno type="doi">10.1007/s11373-004-8184-6</idno><idno type="wicri:Area/PubMed/Corpus">002761</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002761</idno><idno type="wicri:Area/PubMed/Curation">002761</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002761</idno><idno type="wicri:Area/PubMed/Checkpoint">002747</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002747</idno><idno type="wicri:Area/Ncbi/Merge">000F26</idno><idno type="wicri:Area/Ncbi/Curation">000F26</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F26</idno><idno type="wicri:doubleKey">1021-7770:2005:Shao P:development:of:immunoglobulin</idno><idno type="wicri:Area/Main/Merge">004B71</idno><idno type="wicri:Area/Main/Curation">004865</idno><idno type="wicri:Area/Main/Exploration">004865</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.</title><author><name sortKey="Shao, Pei Lan" sort="Shao, Pei Lan" uniqKey="Shao P" first="Pei-Lan" last="Shao">Pei-Lan Shao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pediatrics, National Taiwan University Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name></author><author><name sortKey="Chang, Luan Yin" sort="Chang, Luan Yin" uniqKey="Chang L" first="Luan-Yin" last="Chang">Luan-Yin Chang</name></author><author><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name></author><author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name></author><author><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name></author><author><name sortKey="Huang, Hsiang Yi" sort="Huang, Hsiang Yi" uniqKey="Huang H" first="Hsiang-Yi" last="Huang">Hsiang-Yi Huang</name></author><author><name sortKey="Huang, Fu Yuan" sort="Huang, Fu Yuan" uniqKey="Huang F" first="Fu-Yuan" last="Huang">Fu-Yuan Huang</name></author><author><name sortKey="Lee, Chin Yun" sort="Lee, Chin Yun" uniqKey="Lee C" first="Chin-Yun" last="Lee">Chin-Yun Lee</name></author><author><name sortKey="Chang, Lung Ji" sort="Chang, Lung Ji" uniqKey="Chang L" first="Lung-Ji" last="Chang">Lung-Ji Chang</name></author><author><name sortKey="Wu, T C" sort="Wu, T C" uniqKey="Wu T" first="T-C" last="Wu">T-C Wu</name></author><author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name></author></analytic><series><title level="j">Journal of biomedical science</title><idno type="ISSN">1021-7770</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (immunology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Immunoglobulin G (analysis)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Serologic Tests (methods)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Statistics as Topic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux (immunologie)</term><term>Humains</term><term>Immunoglobuline G (analyse)</term><term>Sensibilité et spécificité</term><term>Statistiques comme sujet</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Test ELISA ()</term><term>Tests sérologiques ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Serologic Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term><term>Statistics as Topic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Sensibilité et spécificité</term><term>Statistiques comme sujet</term><term>Test ELISA</term><term>Tests sérologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chang, Luan Yin" sort="Chang, Luan Yin" uniqKey="Chang L" first="Luan-Yin" last="Chang">Luan-Yin Chang</name><name sortKey="Chang, Lung Ji" sort="Chang, Lung Ji" uniqKey="Chang L" first="Lung-Ji" last="Chang">Lung-Ji Chang</name><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name><name sortKey="Huang, Fu Yuan" sort="Huang, Fu Yuan" uniqKey="Huang F" first="Fu-Yuan" last="Huang">Fu-Yuan Huang</name><name sortKey="Huang, Hsiang Yi" sort="Huang, Hsiang Yi" uniqKey="Huang H" first="Hsiang-Yi" last="Huang">Hsiang-Yi Huang</name><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name><name sortKey="Lee, Chin Yun" sort="Lee, Chin Yun" uniqKey="Lee C" first="Chin-Yun" last="Lee">Chin-Yun Lee</name><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name><name sortKey="Wu, T C" sort="Wu, T C" uniqKey="Wu T" first="T-C" last="Wu">T-C Wu</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Shao, Pei Lan" sort="Shao, Pei Lan" uniqKey="Shao P" first="Pei-Lan" last="Shao">Pei-Lan Shao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004865 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004865 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15864739
|texte= Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15864739" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |